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Micromass UK Limited masslynx software maximum entropy analysis maxent1
Masslynx Software Maximum Entropy Analysis Maxent1, supplied by Micromass UK Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maximum+entropy+software/maxent3/pmc11475375__SMLL___17___2100472___s005-200-7-6
Average 90 stars, based on 1 article reviews
masslynx software maximum entropy analysis maxent1 - by Bioz Stars, 2026-09
90/100 stars

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Article Title: The palmitoyl groups of lung surfactant protein C reduce unfolding into a fibrillogenic intermediate.
Article Snippet: 0022-2836/01/040937±14 $35.00/0 Lung surfactant protein C (SP-C) is a lipophilic peptide that converts from a monomeric a-helical state into b-sheet conformation and forms amyloid ®brils, a process which appears to be accelerated by removal of its two S-palmitoyl groups, and elevated amounts of non-palmitoylated SP-C are found in pulmonary alveolar proteinosis.. Here, we used mass spectrometry to study the ®rst step in ®brillogenesis of di-, monoand non-palmitoylated SP-C. First, the individual decreases in concentration of monomeric a-helical forms of the three peptides in an acidi®ed aqueous organic solvent mixture were monitored by electrospray (ES) mass spectrometry.. Dipalmitoylated SP-C disappeared with a ®rst-order rate constant of 0.01 hÿ1, corresponding to a t1/2 of 70 hours, while SP-C missing one or two palmitoyl groups disappeared with a rate constant of 0.02 hÿ1, t1/2 35 hours.

Article Title: N-terminally extended surfactant protein (SP) C isolated from SP-B-deficient children has reduced surface activity and inhibited lipopolysaccharide binding.
Article Snippet: In both humans and mice, a deficiency of surfactant protein B (SP-B) is associated with a decreased concentration of mature SP-C and accumulation of a larger SP-C peptide, denoted SP-Ci, which is not observed under normal conditions.. Isolation of hydrophobic polypeptides from the lungs of children who died with two different SP-B mutations yielded pure SP-Ci and showed only trace amounts of mature SP-C.. Determination of the SP-Ci covalent structure revealed a 12-residue N-terminal peptide segment, followed by a 35-residue segment that is identical to mature SP-C.

Article Title: Characterization of the binding site for inhibitors of the HPV11 E1-E2 protein interaction on the E2 transactivation domain by photoaffinity labeling and mass spectrometry.
Article Snippet: An indandione-containing class of inhibitors abrogates DNA replication of human papillomavirus (HPV) types 6 and 11 by binding reversibly to the transactivation domain (TAD) of the viral E2 protein and inhibiting its interaction with the viral E1 helicase.. To locate the binding site of this class of protein-protein interaction inhibitors, a benzophenone derivative was used to generate an irreversibly labeled E2-TAD polypeptide.. The single site of covalent modification of the E2-TAD was identified by proteolytic digestions using trypsin, LysC, and V8 proteases and characterization of the resulting peptides by LC-MS procedures.

Article Title: Discordant helix stabilization for prevention of amyloid formation
Article Snippet: .. The ion currents corresponding to the summed abundance of singly and multiply, e.g., doubly and triply protonated molecules, can be determined using maximum entropy software (Micromass). ..

Article Title: Methylation and carbamylation of human ?-crystallins
Article Snippet: .. Resolution of mass spectra of proteins was enhanced using maximum entropy software (Micromass). .. Analysis of peptides by MALDI Enzymatic digests were also analyzed by MALDI MS (Voyager-DE Pro mass spectrometer, Applied Biosystems).

Article Title: Monitoring recombinant protein drugs: a study of insulin by H/D exchange and electrospray ionization mass spectrometry.
Article Snippet: The increasing emergence of new proteinand peptidebased drugs makes necessary the development of rapid and sensitive methods to check consistency between and within batches of biotechnology pharmaceuticals to ensure product quality.. We evaluated electrospray ionization mass spectrometry in combination with H/D isotopic exchange as a potential tool, taking as examples for this case study the four insulins used for treating insulindependent diabetes.. Two (bovine and porcine) are produced naturally, and two are produced by recombinant biotechnology techniques [recombinant human (r-human) and its human insulin analog (LysPro)].

Article Title: Structural and functional consequences of inactivation of human glutathione S-transferase P1-1 mediated by the catechol metabolite of equine estrogens, 4-hydroxyequilenin.
Article Snippet: The inactivation mechanism(s) of human glutathione S-transferase P1-1 (hGST P1-1) by the catechol metabolite of Premarin estrogens, 4-hydroxyequilenin (4-OHEN), was (were) studied by means of site-directed mutagenesis, electrospray ionization mass spectrometric analysis, titration of free thiol groups, kinetic studies of irreversible inhibition, and analysis of band patterns on nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).. The four cysteines (Cys 14, Cys 47, Cys 101, and Cys 169 in the primary sequence) in hGST P1-1 are susceptible to electrophilic attack and/or oxidative damage leading to loss of enzymatic activity.. To investigate the role of cysteine residues in the 4-OHEN-mediated inactivation of this enzyme, one or a combination of cysteine residues was replaced by alanine residues (C47A, C101A, C47A/C101A, C14A/C47A/C101A, and C47A/C101A/C169A mutants).



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